Design of Comb-shaped Surface Electrode to Measure Signal from Tissue Cultured with Electric Stimulation
نویسندگان
چکیده
The comb-shaped surface electrode has been designed to measure the signal from the tissue cultured with electric stimulation. The pattern of the thin film of the surface electrode was made of indium tin oxide (ITO) coated on the glass by the photolithography technique. C2C12 (mouse myoblast cell line) was cultured for 13 days on the patterned scaffold. The electric pulses (period 1 s, amplitude 10 V, duration 0.002 s) were applied for 10 minutes per day. The experimental results show that myoblasts make orientation along the comb-shaped surface electrode, which can be observed through the transparent surface electrode by the optical microscope. The change of the electric resistance of the cultured tissue is able to be detected between electrodes of the experimental system in vitro.
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